DNA

Part:BBa_K4343065

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-08)


SCP2-down

SCP2-down is 1.5kb downstream scp2 site of Y. lipoltiyca, which is part of the donor DNA, allowing for the homologous recombination to integrate various ∆-9 elongase and C16/18 elongase gene of different species with different strength of promoter, hence verifying their contributions to EPA production and further optimizing our engineered strain based on the results of the study.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 522
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1157
    Illegal BsaI.rc site found at 1541


[edit]
Categories
Parameters
None